MOESM1 of Anther culture in rice proportionally rescues microspores according to gametophytic gene effect and enhances genetic study of hybrid sterility

Additional file 1: Figure S1. Chromosomal positions of O. glaberrima fragments in GIL31 (a) and GIL27 (b). Boxes represent chromosomal segments of GIL31 and GIL27 based on genotype data obtained from Oryzabase ( https://shigen.nig.ac.jp/rice/oryzabase/ ). The different colored regions indicate homozygous Taichung65 fragments (white), homozygous IRGC104038 fragments (blue), and heterozygous or undetermined fragments (gray). The black bars on the right sides of boxes indicate homozygous IRGC104038 fragments on chromosome 6 of GIL31 (a) and chromosome 3 of GIL27 (b) harboring S1 and S19, respectively. Figure S2. Graphical representation of genotypes of calli derived from interspecific F1 hybrids between O. sativa and O. glaberrima. A total of 104 anther culture-induced calli were evaluated using 11 simple sequence repeat (SSR) markers linked to HS loci; 52 calli were completely homozygous at all markers, and 52 exhibited partial heterozygosity. Cell colors indicate allele genotypes at the 11 SSR loci as follows: yellow, homozygous for the glaberrima allele; green, homozygous for the sativa allele; red, heterozygous.