Fig 3.TIF (405.49 kB)
Luciferase activity driven by the human pIII promoter in CAL-1 cells.
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posted on 2016-04-22, 06:26 authored by Ryosuke Miura, Kazumi Kasakura, Nobuhiro Nakano, Mutsuko Hara, Keiko Maeda, Ko Okumura, Hideoki Ogawa, Takuya Yashiro, Chiharu NishiyamaA-C. Luciferase assays performed with various lengths of the human pIII promoters (A), the pIII promoters in the presence of PU.1 siRNA (open bar) or control siRNA (closed bar) (B), and the mutant promoters lacking Ets-motif(s) (C). Data represent means ± SEMs of three independent experiments. Each experiment was performed with triplicate samples. *, p < 0.05.
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MHC Class II ExpressionpIII promoter activityMHC class II expressionMHC class II3 rd promoterchromatin immunoprecipitation assaymouse pDCstranscription factorselectrophoretic mobility shift assaysPU .1 bindingmRNA levelsplasmacytoid dendritic cellsPU .1 siRNAGGAAAGAAPU .1 transactivatesCIITAPU .1CALPU .1 knockdownpDC cell linePlasmacytoid Dendritic Cells
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