Loss of FoxO function leads to enhancement of Tsc1 overgrowth under NR.

(A) Eyes bearing eyFlp control, Tsc1 or PTEN mutant clones (marked by the absence of pigmentation), with or without FoxO mutation of animals reared on normal food or NR. Removal of FoxO caused lethality of Tsc1 mutant animals under NR. (B) Eye discs with control, FoxO, Tsc1 or Tsc1 FoxO mutant tissue dissected from larvae reared on normal food or NR; (B’) quantification of eye disc size. Scale bars are 250 μm. (C) Nuclear (DAPI, blue) and cell membrane (phalloidin, red) staining in eye discs with hsFlp control, FoxO, Tsc1 or Tsc1 FoxO clones (marked by the absence of GFP) dissected from larvae reared on NR. Scale bars are 75 μm.