ppat.1006876.g002.tif (870.09 kB)
Localization of B. subtilis membrane proteins after treatment with rhodomyrtone.
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posted on 2018-02-16, 18:42 authored by Dennapa Saeloh, Varomyalin Tipmanee, Kin Ki Jim, Marien P. Dekker, Wilbert Bitter, Supayang P. Voravuthikunchai, Michaela Wenzel, Leendert W. Hamoen(A) B. subtilis strains (see S1 Table for detailed information) were grown until an OD600 of 0.3 and subsequently treated with rhodomyrtone (1x MIC). See also overview pictures of GFP-MreB in S2 and S3 Figs. (B) Time lapse microscopy of DivIVA-GFP. B. subtilis strain HS63 was mounted on an agarose patch, and placed in a flow chamber. Constant medium flow provided sufficient nutrient and oxygen supply during the experiment. Rhodomyrtone was added with the medium flow and pictures were taken every 2 min. See also S1 Movie. Scale bar 2 μm.
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ATP synthaseacylphloroglucinol rhodomyrtoneintracellular proteinelectron microscopymembrane-inserting moleculerhodomyrtone transientlyrhodomyrtone causesAberrant protein localization impairsantibiotic candidatemembrane proteinsform intracellular vesiclesmembrane curvaturebinding modeintracellular targetsmyrtle Rhodomyrtus tomentosacauses distortionform protein-trapping membrane vesiclesmembrane invaginationsPreceding studiesamphipathic structurenovel mechanismmembrane fluidizationnovel antibiotic rhodomyrtone traps membrane proteinsdynamics simulationsphospholipid head groups
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