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Laccase-Catalyzed Oxidation of Iodide and Formation of Organically Bound Iodine in Soils
journal contribution
posted on 2013-01-02, 00:00 authored by Miharu Seki, Jun-ichi Oikawa, Taro Taguchi, Toshihiko Ohnuki, Yasuyuki Muramatsu, Kazunori Sakamoto, Seigo AmachiLaccase oxidizes iodide to molecular iodine or hypoiodous
acid,
both of which are easily incorporated into natural soil organic matter.
In this study, iodide sorption and laccase activity in 2 types of
Japanese soil were determined under various experimental conditions
to evaluate possible involvement of this enzyme in the sorption of
iodide. Batch sorption experiment using radioactive iodide tracer
(125I–) revealed that the sorption was
significantly inhibited by autoclaving (121 °C, 40 min), heat
treatment (80 and 100 °C, 10 min), γ-irradiation (30 kGy),
N2 gas flushing, and addition of reducing agents and general
laccase inhibitors (KCN and NaN3). Interestingly, very
similar tendency of inhibition was observed in soil laccase activity,
which was determined using 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)
(ABTS) as a substrate. The partition coefficient (Kd: mL g–1) for iodide and specific activity
of laccase in soils (Unit g–1) showed significant
positive correlation in both soil samples. Addition of a bacterial
laccase with an iodide-oxidizing activity to the soils strongly enhanced
the sorption of iodide. Furthermore, the enzyme addition partially
restored iodide sorption capacity of the autoclaved soil samples.
These results suggest that microbial laccase is involved in iodide
sorption on soils through the oxidation of iodide.