L* compensates ns2 RNase L antagonist activity in bone marrow-derived macrophages (BMM) and in mice.

A. Schematic diagram of recombinant MHV. B. L2 fibroblasts were infected (1 PFU/cell). At indicated time points post-infection, virus titers in the cell lysates combined with supernatants were determined by plaque assay (n = 3). C-D. BMM, derived from WT or RNase L−/− mice were infected (1 PFU/cell). At indicated time points post-infection, titers of viruses in the cell lysates combined with supernatants were determined by plaque assay (n = 3). E. Four-week-old WT or RNase L−/− B6 mice were inoculated intrahepatically with WT A59, mutant and chimeric viruses (2000 PFU/mouse). At 5 d.p.i., organs were harvested, homogenized and virus titers determined by plaque assay (n = 4 or 5). Statistics were done using the Mann-Whitney test. Error bars represent standard error of the means.