Figure_3.tif (3.05 MB)

LNA-antimiR-34a maintains cardiac function in a moderate model of pressure overload, but not in a severe model.

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posted on 2014-02-27, 04:21 authored by Bianca C. Bernardo, Xiao-Ming Gao, Yow Keat Tham, Helen Kiriazis, Catherine E. Winbanks, Jenny Y. Y. Ooi, Esther J. H. Boey, Susanna Obad, Sakari Kauppinen, Paul Gregorevic, Xiao-Jun Du, Ruby C. Y. Lin, Julie R. McMullen

(A) Graphs of HW/TL, AW/TL and LW/TL in control, TAC moderate (TAC mod) and TAC severe (TAC sev) LNA-control (c) and LNA-antimiR-34a (a) groups. N = 3–8 per group. ***P<0.001 vs. control, **P<0.01 vs. control, *P<0.05 vs. control, †P<0.05, §P<0.05 vs. TAC-moderate of same treatment group. 1 way ANOVA followed by Fisher’s Post Hoc Test (comparing all five groups). When performing 1 way ANOVA followed by Fisher’s Post Hoc Test on control and TAC moderate groups only (i.e. comparing three groups), n = 4–8 per group, ∧P<0.05 vs. control, ‡P<0.05. (B) Representative LV cross-sections stained with wheat germ agglutinin from control and TAC moderate and severe LNA-control and LNA-antimiR-34a mice, and quantification of cell area. Scale bar = 50 µM. Data are expressed as mean ± SEM. N = 3–5 per group. **P<0.01 vs. control, ***P<0.001 vs. control, §P<0.05. 1 way ANOVA followed by Fisher’s Post Hoc Test (comparing all five groups). (C) Quantification of fractional shortening at baseline (pre-surgery), four weeks post TAC (before LNA oligonucleotide administration) and 12 weeks post (i.e. eight weeks post treatment) and representative M-mode echocardiograms. N = 4–5 per group. *P<0.05 vs. baseline of the same groups and control at the same time point, †P<0.05, ‡P<0.05 vs. same group at 4 weeks post TAC, §P<0.05 vs. TAC-moderate at same time point. 1 way ANOVA followed by Fisher’s Post Hoc Test.