LGR4 is a direct target of miR-34a.

(A) HEK293 cells were co-transfected with miR-34a, pMIR-LGR4 3’-UTR or pMIR-LGR4 3’-UTR-Mut, along with a pRL-SV40 reporter plasmid. After 24 hours, the luciferase activity was measured. Values are presented as relative luciferase activity after normalized to Renilla luciferase activity. Results are expressed as mean ± SD. n = 3, *p < 0.05. (B) ARPE-19 cells were transfected with either miR-34a or a negative control (NC). Cell lysates were prepared and used for Western blot analysis of LGR4. β-actin was probed as loading control. The band intensity was measured with ImageJ software. Images are representative of at least three independent experiments.