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Known parkin-interacting proteins do not show detectable association with parkin on native gels

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posted on 2011-12-31, 04:35 authored by Cindy Van Humbeeck, Etienne Waelkens, Olga Corti, Alexis Brice, Wim Vandenberghe

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Taken from "Parkin occurs in a stable, non-covalent, ∼110-kDa complex in brain"

The European Journal of Neuroscience 2008;27(2):284-293.

Published online Jan 2008

PMCID:PMC2253705.

© The Authors (2007). Journal Compilation © Federation of European Neuroscience Societies and Blackwell Publishing Ltd

(A–F) S and P fractions from brain were separated by glycerol gradient centrifugation and BN-PAGE, followed by Western blotting for the protein indicated. The dotted ellipses indicate the position of the parkin band, as determined by reprobing each blot with anti-parkin.

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