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Knockdown of endogenous psyllid mRNAs by dsRNA feeding on artificial diets after 120 h.

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posted on 10.03.2017 by Diogo Manzano Galdeano, Michèle Claire Breton, João Roberto Spotti Lopes, Bryce W. Falk, Marcos Antonio Machado

The dsRNAs were added to the artificial diets at concentrations of 200, 500 and 1000 ng.μL-1, and total RNAs were isolated from live psyllids after 120 h of feeding. GFP dsRNA-fed D. citri served as controls for each experiment. (A) Accumulation of cathepsin D mRNAs in whole psyllids fed cathepsin D dsRNAs at 200, 500 and 1000 ng.μL-1. (B) Accumulation of chitin synthase mRNAs in whole psyllids fed chitin synthase dsRNAs at 200, 500 and 1000 ng.μL-1. (A) Accumulation of inhibitor of apoptosis mRNAs in whole psyllids fed inhibitor of apoptosis dsRNAs at 200, 500 and 1000 ng.μL-1. Significant differences in relative expression were evaluated between test dsRNAs and GFP dsRNA at the same concentrations. A single asterisk indicates P<0.05, and double asterisks indicate P<0.01.

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