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Isolation of Protein Ligands from Large Peptoid Libraries
journal contribution
posted on 2003-10-24, 00:00 authored by Prasanna G. Alluri, M. Muralidhar Reddy, Kiran Bachhawat-Sikder, Hernando J. Olivos, Thomas KodadekThe isolation of ligands for large numbers of proteins is an important goal in proteomics. Whereas
peptide libraries are rich sources of protein-binding molecules, native peptides have certain undesirable
properties, such as sensitivity to proteases that make them less than ideal for some applications. We report
here the construction and characterization of large, chemically diverse combinatorial libraries of peptoids
(N-substituted oligoglycines). A protocol for the isolation of specific protein-binding molecules from these
libraries is described. These data suggest that peptoid libraries will prove to be inexpensive and convenient
sources of protein ligands.