Isolation and purification of orientin and vitexin from <i>Trollius chinensis</i> Bunge by high-speed counter-current chromatography

<div><p>Orientin and vitexin are the two main bioactive compounds in <i>Trollius chinensis</i> Bunge. In this study, a rapid method was established for the isolation and purification of orientin and vitexin from <i>T. chinensis</i> Bunge using high-speed counter-current chromatography in one step, with a solvent system of ethyl acetate–ethanol–water (4:1:5, v/v/v). A total of 9.8 mg orientin and 2.1 mg vitexin were obtained from 100 mg of the ethyl acetate extract, with purities of 99.2% and 96.0%, respectively. Their structures were identified by UV, MS and NMR. The method was efficient and convenient, which could be used for the preparative separation of orientin and vitexin from <i>T. chinensis</i> Bunge.</p></div>