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Investigation of the morphology of symbiotic nodule using phage-displayed scFv clone bDOA9rb8.

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posted on 2017-06-27, 17:41 authored by Nguyen Xuan Vu, Natcha Pruksametanan, Witsanu Srila, Watcharin Yuttavanichakul, Kamonluck Teamtisong, Neung Teaumroong, Nantakorn Boonkerd, Panlada Tittabutr, Montarop Yamabhai

Bright field and immunofluorescence staining images from confocal microscopy of bacteriod form of Bradyrhizobium sp. DOA9 in plant nodules at different magnifications are shown. Sample in A was detected with negative phage clone 3C1; B rabbit polyclonal antibody, or C the phage-displayed scFv clone bDOA9rb8 from this study. Blue color indicated the plate cell wall that was stained with blue fluorophore. Scale bar is 100 μm at 10X magnification and 50 μm at 40X magnifications.

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