Interaction of LANA with PRC2.

(A) Gel filtration chromatography of nuclear extracts derived from iSLK and iSLKBAC16 cells. Fractions from 28 to 72 were tested by immunoblot analysis for LANA and polycomb proteins. Molecular weight markers are indicated. (B) Co-immunoprecipitation of LANA-V5 (using V5 antibody) from 293T cells expressing LANA-V5 and the indicated 3xFLAG-tagged PRC2 (EZH2, SUZ12 and EED) or PRC1 (RING1B and BMI-1) factors. Immunoprecipitation and input blots were probed with V5 and FLAG antibodies. (C) Cell lysates derived from de novo KSHV infected iSLK cells (72 hpi) were used for immunoprecipitation with anti-EZH2 rabbit antibody (αEZH2) or normal rabbit IgG (αIgG), followed by immunoblotting with αLANA and αEZH2.