Inhibitor screen for epigenetic regulatory proteins that induce KSHV reactivation from latency.

<p>BJAB-KSHV.219 cells were incubated with 1 μM of each compound for 48 hours in (A) or 10 μM of each compound for 120 hours in (B), and the percentage of GFP positive cells expressing RFP (percent reactivation) was determined by FACS analysis. (C) BJAB-KSHV.219 cells were incubated with EZH2 inhibitors at concentrations of 50, 16.7, 5.6, and 1.9 μM for 120 hours. Percent reactivation was determined by FACS analysis. Data are representative of two independent experiments performed in duplicate; mean and standard error are shown.</p>