Inhibition of odorant and Orco agonist initiated current responses of oocytes expressing insect odorant receptors by various amines

2014-04-07T08:21:43Z (GMT) by Sisi Chen Charles W. Luetje
<p>Figure 2C Data. Current responses of oocytes expressing Cqui\Orco to 30µM OLC12 (~EC5) in the presence of 100µM of each compound presented as a percentage of the average of two preceding responses to OLC12 alone. Sham is an application of ND96 (no antagonist candidate).<br>Figure 3A Data. Current responses of oocytes expressing Cqui\Orco to 30µM OLC12 (~EC5) in the presence of various concentrations of tryptamine, tyramine or phenethylamine are presented as a percentage of the response to OLC12 alone.</p> <p>Figure 3B Data_30µM OLC12 curve. Current responses of oocytes expressing Cqui\Orco to 30µM OLC12 in the presence of various concentrations of tryptamine are presented as a percentage of the response to OLC12 alone.</p> <p>Figure 3B Data_100µM OLC12 curve. Current responses of oocytes expressing Cqui\Orco to 100µM OLC12 in the presence of various concentrations of tryptamine are presented as a percentage of the response to OLC12 alone.</p> <p>Figure 4A Data. Oocytes expressing Cqui\Orco, Agam\Orco or Dmel\Orco were activated by OLC12 (Cqui\Orco, 30µM; Agam\Orco, 30µM; Dmel\Orco, 20µM). Values are the current responses in the presence of 10µM tryptamine presented as a percentage of the average of two preceding responses to OLC12 alone.</p> <p>Figure 4B Data. Oocytes expressing Agam\Orco+Agam\Or65 were activated by 100nM eugenol, oocytes expressing Onub\Orco+Onub\Or6 were activated by 1µM Z11-14:OAc, oocytes expressing Mcar\Orco+Mcar\Or5 were activated by 150µM 2-phenylethanol. Values represent the normalized (see Methods) percentage of current responses in the presence of 10µM tryptamine to the preceding response to odorant alone.</p> <p>Figure 4C Data. Oocytes expressing Agam\Orco+Agam\Or65 were activated by 100nM eugenol, oocytes expressing Onub\Orco+Onub\Or6 were activated by 1µM Z11-14:OAc, oocytes expressing Mcar\Orco+Mcar\Or5 were activated by 150µM 2-phenylethanol. Values represent the normalized (see Methods) percentage of current responses in the presence of 100µM tyramine to the preceding response to odorant alone.</p> <p>Figure 5A Data_OLC12 20µM curve. Current responses of oocytes expressing Agam\Orco+Agam\Or65 to 20µM OLC12 in the presence of various concentrations of tryptamine are presented as a percentage of the response to OLC12 alone.</p> <p>Figure 5A Data_OLC12 100µM curve. Current responses of oocytes expressing Agam\Orco+Agam\Or65 to 100µM OLC12 in the presence of various concentrations of tryptamine are presented as a percentage of the response to OLC12 alone.</p> <p>Figure 5B Data_Eugenol 10nM curve. Oocytes expressing Agam\Orco+Agam\Or65 were activated by 10nM eugenol. Values are the normalized (see Methods) percentage of current responses in the presence of various concentrations of tryptamine compared to the preceding response to odorant alone.</p> <p>Figure 5B Data_Eugenol 100nM curve. Oocytes expressing Agam\Orco+Agam\Or65 were activated by 100nM eugenol. Values are the normalized (see Methods) percentage of current responses in the presence of various concentrations of tryptamine compared to the preceding response to odorant alone.</p> <p>Figure 6A Data. Values represent the normalized (see Methods) percentage of current responses of oocytes expressing Agam\Orco+Agam\Or27 (activated by 3µM L-fenchone), Agam\Orco+Agam\Or28 (activated by 40µM acetophenone), Agam\Orco+Agam\Or31 (activated by 70µM geranyl acetate), Agam\Orco+Agam\Or39 (activated by 10µM 6-methyl-5-hepten-2-one), Agam\Orco+Agam\Or48 (activated by 3µM 2-nonanone), or Agam\Orco+Agam\Or65 (activated by 100nM eugenol) in the presence of 10µM tryptamine, as compared to the preceding response to odorant alone.</p> <p>Figure 6B Data. Values represent the normalized (see Methods) percentage of current responses of oocytes expressing Agam\Orco+Agam\Or27 (activated by 3µM L-fenchone), Agam\Orco+Agam\Or28 (activated by 40µM acetophenone), Agam\Orco+Agam\Or31 (activated by 70µM geranyl acetate), Agam\Orco+Agam\Or39 (activated by 10µM 6-methyl-5-hepten-2-one), Agam\Orco+Agam\Or48 (activated by 3µM 2-nonanone), or Agam\Orco+Agam\Or65 (activated by 100nM eugenol) in the presence of 10µM tyramine, as compared to the preceding response to odorant alone.</p> <p> </p>