Induction of mitotic arrest by DATS through the inhibition of mitotic spindle formation

Detection of Ser10-phosphorylated histone H3, a sensitive marker for cells at the M phase, was detected in HT-29 cells cultured with DATS (10 μM) for 12 h by using a flow cytometer (panel ). Spindle formation of HT-29 cells cultured with vehicle or DATS (10 μM) was assessed by the immunofluorescence method using anti-β-tubulin antibody, as described in Materials and Methods (green; panel ). The nucleus was counterstained with propidium iodide (magenta). Scale bar 20 μm.

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Taken from "Alkenyl group is responsible for the disruption of microtubule network formation in human colon cancer cell line HT-29 cells"

Carcinogenesis 2008;29(7):1400-1406.

Published online 29 May 2008

PMCID:PMC2500214.