Improvement of the activity and thermostability of microbial transglutaminase by multiple-site mutagenesis

<p>Microbial transglutaminase (MTG) is an enzyme widely used in the food industry. Mutiple-site mutagenesis of <i>Streptomyces mobaraensis</i> transglutaminase was performed in <i>Escherichia coli</i>. According to enzymatic assay and thermostability study, among three penta-site MTG mutants (DM01-03), DM01 exhibited the highest enzymatic activity of 55.7 ± 1.4 U/mg and longest half-life at 50 °C (418.2 min) and 60 °C (24.8 min).</p>