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Impact of the genotype for acid resistance patterns of pathogenic and probiotic -3

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posted on 2011-12-31, 04:06 authored by Sina M Coldewey, Maike Hartmann, Dorothea S Schmidt, Uta Engelking, Sya N Ukena, Florian Gunzer

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Taken from "Impact of the genotype for acid resistance patterns of pathogenic and probiotic "

BMC Microbiology 2007;7():21-21.

Published online 26 Mar 2007

PMCID:PMC1852560.

were constructed using low copy vector pBR322 as backbone. Each of them harbors one complete gene and the p promoter [39] from EHEC/STEC strains 126814 (pSC1), EDL933(pSC2), EDL933(pUD2), 86-24 (pUD4), E-D53 (pUD8) and 288597 (pUD10), amplified by PCR with primers RpoS 8/RpoS 9 containing 5' III restriction sites. RpoS 8 and RpoS 4c were taken to synthesize the respective DNA fragment from STEC E-D68 in order to generate the two independent plasmids pUD6 and pUD9. pDS4 contained the cloned gene from EcN, amplified with primers RpoS 8 and RpoS 22. pMH33 was made from pSC2 by PCR mediated site specific mutagenesis, employing the mutation to resolve the TAA stop codon in *. RpoS negative mutant strain MHH126-5 was transformed with each of the complementation vectors by electroporation.