Impact of increase in concentration of oligomers on formation of inter and intramolecular G-quadruplex species.

The wild type DNA sequence spanning region I of HOX11 breakpoint region, MN38 (G-rich strand) or MS113 (mutant) was used for titration. MN37, the C-rich strand was used as a negative control. (A) EMSA gel shows formation of inter (boxed grey) and intramolecular G-quadruplexes. The intramolecular G-quadruplexes were further divided into species with (boxed blue) or without (boxed red) GNG motifs. (B) Bar diagram shows quantification of inter and intramolecular species over a range of oligonucleotide concentrations.