Fig 5.tif (3.25 MB)
Immunolabeling of MCF7 cells in suspension expressing the fusion protein ErbB2-TagRFP.
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posted on 2017-03-17, 17:29 authored by Andrés Martín Toscani, Rocío G. Sampayo, Federico Martín Barabas, Federico Fuentes, Marina Simian, Federico Coluccio LeskowMCF7 cells expressing the fusion protein ErbB2-TagRFP in suspension were immunolabeled for beta1 integrin. 3D-reconstruction allows us to determine that both proteins coexist in conspicuous domains in the surface of the cell membrane. This is a representative image of 20 acquired cells. All cells showed clusters with positive colocalization (r > 0.5). Scale bar: 5um.
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breast cancer cellsErbB 2 overexpressionErbB 2 membrane domainsextracellular matrix proteins shape membrane clusters distributionextracellular matrix proteinsDistinct ErbB 2 receptor populations differentiallyplasma membraneErbB 2beta 1 integrinErbB 2 formstyrosine kinase receptorsErbB 2 colocalizationbreast cancer cell models ErbB 2beta 1 integrin activityexpression levelsbreast cancer progression
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