Fig 4.tif (7 MB)
Immunofluorescence staining for extracellular matrix proteins in day 1 and day 7 HWJSC spheroids.
figure
posted on 2017-09-12, 17:32 authored by David G. Belair, Cynthia J. Wolf, Carmen Wood, Hongzu Ren, Rachel Grindstaff, William Padgett, Adam Swank, Denise MacMillan, Anna Fisher, Witold Winnik, Barbara D. AbbottHWJSC spheroids were cultured for either 1 day or 7 days (with 6 days of osteogenic differentiation), fixed, and stained for extracellular matrix proteins: collagen I (A) collagen IV (B), laminin (C), and fibronectin (D) and counter-staining with Hoechst. Scale bars represent 100 μm.
