Human α-lactalbumin gene expression in GMECs in vitro.

(A) Shematic overview dipicting position of primers Bc-F/Bc-R and Hc-F/Hc-R on BLG and hLA exons respectively. Blue boxes, exons of BLG or hLA; Level arrow lines, primers used for RT-PCR. (B) Reverse-transcription PCR analysis of partial BLG (i), hLA (ii) and GAPDH (iii) mRNAs in mono- and bi-allelic BLG-targeted cell clones. NC: non-transfected cells; m1, m2, m3: mono-allelic BLG-targeted clones; b1, b2, b3: bi-allelic BLG-targeted cell clones. (C) qPCR analysis of BLG and hLA expression. WT: non-transfected cells; m1, m2, m3: mono-allelic BLG-targeted clones; b1, b2, b3: bi-allelic BLG-targeted cell clones. The results were derived from at least three different experiments, P<0.05. (D) Western blot analysis for hLA in the supernatants of transfected GMECs. Lane WT, supernatant of non-transfected cells; Lanes 1–2, supernatant of bi-allelic and mono-allelic BLG-targeted cell clones.