Host tissue colonization by the parasite following a tsetse mediated transmission.

Numbers of parasites in the ears, draining lymph nodes and peripheral blood of mice exposed to SG+ tsetse fly bites as determined by volumetric flow cytometry detecting the dsRed transgene (A) and by SL-RNA specific RT-qPCR (B). Data shown are from three individual mice for each time point. Each ear was exposed to the bites of an individual infected tsetse fly. No statistical significant differences were recorded between the two different quantification methods. These parasite kinetics data are representative of two independent experiments. Data are shown as the mean ± SEM. *: P <0.05