ja505278w_si_001.pdf (1.14 MB)
Highly Selective Fluorogenic Multianalyte Biosensors Constructed via Enzyme-Catalyzed Coupling and Aggregation-Induced Emission
journal contribution
posted on 2014-07-16, 00:00 authored by Xiaorui Wang, Jinming Hu, Guoying Zhang, Shiyong LiuThe development of a highly selective
and fast responsive fluorogenic
biosensor for diverse analytes ranging from bioactive small molecules
to specific antigens is highly desirable but remains a considerable
challenge. We herein propose a new approach by integrating substrate-selective
enzymatic reactions with fluorogens exhibiting aggregation-induced
emission feature. Tyrosine-functionalized tetraphenylethene, TPE-Tyr,
molecularly dissolves in aqueous media with negligible fluorescence
emission; upon addition of horseradish peroxidase (HRP) and H2O2, effective cross-linking occurs due to HRP-catalyzed
oxidative coupling of tyrosine moieties in TPE-Tyr. This leads to
fluorescence emission turn-on and fast detection of H2O2 with high sensitivity and selectivity. As a validation of
the new strategy’s generality, we further configure it into
the biosensor design for glucose through cascade enzymatic reactions
and for pathologically relevant antigens (e.g., human carcinoembryonic
antigen) by combining with the ELISA kit.