HPTLC analysis of <i>Scoparia dulcis</i> Linn (Scrophulariaceae) and its larvicidal potential against dengue vector <i>Aedes aegypti</i>

<div><p>This study evaluates the larvicidal activity of <i>Scoparia dulcis</i> aqueous extract against dengue vector and determines its major chemical components. The extract was tested at various concentrations ranging from 0.1 to 2 mg/mL against <i>Aedes aegypti</i> larvae. The extracts displayed significant larvicidal efficacy against <i>Ae. aegypt</i> species after 24 h exposure revealing LC<sub>50</sub> of 3.3835 (mg/mL) and LC<sub>90</sub> of 5.7578 (mg/mL). Finger printing profile carried out by CAMAG automatic TLC sample applicator programmed through WIN CATS software revealed peaks with different <i>R</i><sub>f</sub> values for three different volumes injected: 16, 15 and 18 peaks were spotted for 3, 6 and 9 μL, respectively. Ascending order of <i>R</i><sub>f</sub> values was also ascertained for each peak recorded. This study clearly signifies that <i>S. dulcis</i> extract contains numerous compounds that are known to have larvicidal properties which clearly substantiates its efficacy on <i>Ae. aegypti</i> larvae.</p></div>