HCV NS5A colocalizes with LARP1 in infected Huh7.5 cells.

A. Cells were infected by HCV over 4 d prior to fixation, NS5A and LARP1 double-staining, and confocal microscopy. Nuclei were counterstained with Hoechst 33342. LARP1 (red) and NS5A (green) were detected using Alexa-594 and Alexa-488, respectively. Overlays were generated by the ImageJ software. Solid arrow shows colocalization site. Bar = 15 μm. B. Statistical assessment of the colocalization of LARP1 and NS5A. Red (LARP1) and green (NS5A) fluorescence intensities were measured for each pixel along a 5-μm horizontal line centered around the arrow tip using the Plot Profile function of the ImageJ software. Spearman correlation coefficients for each couple of intensity values are shown. The underlying data for panels in this figure can be found in S1 Data.