Gross phenotype of Spink5- Klk- deficient mutant lines.

<p><b>(A)</b> Phenotype of wt, Sp5<sup>A135X/A135X</sup>, Klk5<sup>-/-</sup>Sp5<sup>A135X/A135X</sup>, Klk7<sup>-/-</sup>Sp5<sup>A135X/A135X</sup> and Klk5<sup>-/-</sup>Klk7<sup>-/-</sup>Sp5<sup>A135X/A135X</sup> mice 12 hours after birth. Peeling skin was observed in Sp5<sup>A135X/A135X</sup> and Klk7<sup>-/-</sup>Sp5<sup>A135X/A135X</sup> and to lesser extent in Klk5<sup>-/-</sup>Sp5<sup>A135X/A135X</sup> pups (black arrowheads). <b>(B)</b> wt, Klk5<sup>-/-</sup>Sp5<sup>A135X/A135X</sup>, and Klk5<sup>-/-</sup>Klk7<sup>-/-</sup>Sp5<sup>A135X/A135X</sup> mice at P5. Klk5<sup>-/-</sup>Sp5<sup>A135X/A135X</sup> pups showed dry, scaly skin while Klk5<sup>-/-</sup>Klk7<sup>-/-</sup>Sp5<sup>A135X/A135X</sup> mice had stretched and shiny epidermis, with no visual signs of dehydration. <b>(C)</b> wt and Klk5<sup>-/-</sup>Klk7<sup>-/-</sup>Sp5<sup>A135X/A135X</sup> mice at 3 weeks. Klk5<sup>-/-</sup>Klk7<sup>-/-</sup>Sp5<sup>A135X/A135X</sup> mice showed alopecia and growth retardation. <b>(D)</b> wt and Klk5<sup>-/-</sup>Klk7<sup>-/-</sup>Sp5<sup>A135X/A135X</sup> mice at 6 weeks. <b>(E)</b> Vibrissae hair obtained from P5 pups analysed by scanning electron microscopy. Klk5<sup>-/-</sup>Klk7<sup>-/-</sup>Sp5<sup>A135X/A135X</sup> showed hair shaft abnormalities similar to bamboo hair; Scale bar, 30 μm. <b>(F)</b> Progression of body weight of wt, Klk5<sup>-/-</sup>Sp5<sup>A135X/A135X</sup>, Klk5<sup>-/-</sup>Klk7<sup>-/-</sup>Sp5<sup>A135X/A135X</sup> and Klk5<sup>-/-</sup>Klk7<sup>-/-</sup> mice, n>5, error bars represent standard errors of mean.</p>