pgen.1006566.g003.tif (2.59 MB)

Gross phenotype of Spink5- Klk- deficient mutant lines.

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posted on 2017-01-17, 17:41 authored by Petr Kasparek, Zuzana Ileninova, Olga Zbodakova, Ivan Kanchev, Oldrich Benada, Karel Chalupsky, Maria Brattsand, Inken M. Beck, Radislav Sedlacek

(A) Phenotype of wt, Sp5^A135X/A135X, Klk5^-/-Sp5^A135X/A135X, Klk7^-/-Sp5^A135X/A135X and Klk5^-/-Klk7^-/-Sp5^A135X/A135X mice 12 hours after birth. Peeling skin was observed in Sp5^A135X/A135X and Klk7^-/-Sp5^A135X/A135X and to lesser extent in Klk5^-/-Sp5^A135X/A135X pups (black arrowheads). (B) wt, Klk5^-/-Sp5^A135X/A135X, and Klk5^-/-Klk7^-/-Sp5^A135X/A135X mice at P5. Klk5^-/-Sp5^A135X/A135X pups showed dry, scaly skin while Klk5^-/-Klk7^-/-Sp5^A135X/A135X mice had stretched and shiny epidermis, with no visual signs of dehydration. (C) wt and Klk5^-/-Klk7^-/-Sp5^A135X/A135X mice at 3 weeks. Klk5^-/-Klk7^-/-Sp5^A135X/A135X mice showed alopecia and growth retardation. (D) wt and Klk5^-/-Klk7^-/-Sp5^A135X/A135X mice at 6 weeks. (E) Vibrissae hair obtained from P5 pups analysed by scanning electron microscopy. Klk5^-/-Klk7^-/-Sp5^A135X/A135X showed hair shaft abnormalities similar to bamboo hair; Scale bar, 30 μm. (F) Progression of body weight of wt, Klk5^-/-Sp5^A135X/A135X, Klk5^-/-Klk7^-/-Sp5^A135X/A135X and Klk5^-/-Klk7^-/- mice, n>5, error bars represent standard errors of mean.