ac0103221_si_001.pdf (50.08 kB)
Gold Nanoparticle-Based Quantitative Electrochemical Detection of Amplified Human Cytomegalovirus DNA Using Disposable Microband Electrodes
journal contribution
posted on 2001-08-10, 00:00 authored by Laurent Authier, Céline Grossiord, Pierre Brossier, Benoît LimogesAn electrochemical DNA detection method has been
developed for the sensitive quantification of an amplified
406-base pair human cytomegalovirus DNA sequence
(HCMV DNA). The assay relies on (i) the hybridization of
the single-stranded target HCMV DNA with an oligonucleotide-modified Au nanoparticle probe, (ii) followed by the
release of the gold metal atoms anchored on the hybrids
by oxidative metal dissolution, and (iii) the indirect
determination of the solubilized AuIII ions by anodic
stripping voltammetry at a sandwich-type screen-printed
microband electrode (SPMBE). Due to the enhancement
of the AuIII mass transfer by nonlinear diffusion during
the electrodeposition time, the SPMBE allows the sensitive determination of AuIII in a small volume of quiescent
solution. The combination of the sensitive AuIII determination at a SPMBE with the large number of AuIII released
from each gold nanoparticle probe allows detection of as
low as 5 pM amplified HCMV DNA fragment.
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nanoparticle probeAmplified Human Cytomegalovirus DNAnonlinear diffusionIII mass transfergold nanoparticle probe5 pMcytomegalovirus DNA sequenceSPMBEoxidative metal dissolutionDisposable Microband ElectrodesIII ionsHCMV DNAelectrochemical DNA detection methodelectrodeposition timeHCMV DNA fragmentIII determinationgold metal atoms
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