GlCHC reporters as seen by gSTED microscopy, standard LSCM and co-labeled with dextran-TxR.

(A, B) The improved resolution of gSTED super resolution microscopy (green signal) compared to a standard LSCM signal (red signal) of the HA-tagged GlCHC reporter in fixed cells indicates that GlCHC assembly size is in the range of 50nm (A: inset of B). (C-E) Single optical sections of GFP-tagged GlCHC-reporters (C) co-labeled with the fluid phase marker dextran-TxR (D) (E: merged images). (F, G) Surface rendering of optical sections from the cell shown in C-E shows that the GlCHC-GFP reporter (green) localizes distal to the PV-marker dextran-TxR (F: ventral view, G: dorso-lateral view).