Gkl628f1.jpg (26.33 kB)
Genomically integrated cassettes for constitutive or Tet-inducible expression of shRNA/Plk1 or shRNA/Plk1MM
figure
posted on 2011-12-31, 02:53 authored by Sven Kappel, Yves Matthess, Brigitte Zimmer, Manfred Kaufmann, Klaus StrebhardtCopyright information:
Taken from "Tumor inhibition by genomically integrated inducible RNAi-cassettes"
Nucleic Acids Research 2006;34(16):4527-4536.
Published online 31 Aug 2006
PMCID:PMC1636372.
© 2006 The Author(s)
() Different promoter derivatives for constitutive or Tet-inducible expression of shRNA/Plk1 or shRNA/Plk1MM were stably integrated into TetR-expressing HeLa cells followed by isolation of single cell clones: wild-type H1 promoter for constitutive expression of mismatch shRNA, US-type promoter containing TetO upstream of the TATA box; DS-type promoter containing TetO downstream of the TATA box and the US/DS-type promoter containing TetO upstream and TetO downstream of the TATA box. TetO, tetracycline operon; TATA, TATA box. () Schematic representation of promoter function in absence (−Dox) and presence of doxycycline (+Dox).