Generation of mice with prostate-specific Lzts2 and Pten deletion.

(A) Schematic representation of Lzts2 and Pten compound mice. On chromosome 19, loxP sites flank exon 5 of the pten gene and exons 2 and 3 of the lzts2 gene. Crossing with PB-Cre mice results in prostate-specific recombination of the loxP sites and removal of these exons from this locus. (B) Genomic PCR analysis of Lzts2 and Pten alleles in prostate tissues using specific primers as detailed in (A). (C) Histological and immunohistochemical analysis of 6–8 month old Lzts2^LoxP/LoxP:PB-Cre4, Pten^loxP/Wt:PB-Cre4, and Lzts2^LoxP/LoxP -Pten^loxP/Wt:PB-Cre4 mice. Top panel depicts H&E staining of prostate tissues. Immunohistochemistry of Lzts2 (middle) and Pten (lower) on sequential sections are shown below.