GBP1 and GBP2 are downstream of TOR signaling.
(A, B) Overexpressing either GBP1 or GBP2 partially rescues body size reduction in females (A) and males (B) with reduced TOR signaling in their fat bodies. n = 47–64 for A and 59–67 for B. (C) Overexpressing either GBP1 or GBP2 partially increases growth rate in larvae with reduced TOR signaling in the fat body. n = 14-18/time point. (D) Overexpressing either GBP1 or GBP2 partially rescues the duration of the L3 in TOR signaling reduced larvae. n = 108–129. (E, F) Overexpressing either GBP1 or GBP2 reduces ILP2 (E) and ILP5 (F) accumulation in the insulin-producing cells. (G, H) Overexpressing GBP1 or GBP2 reduces the densities of ILP2 (G) and ILP5 (H) signals in the insulin-producing cells. We standardized the densities of ILPs by fixing the values from C7>GFP, TSC1, TSC2 to 1. n = 30–42 for both ILP2 and ILP5. (I) Overexpressing either GBP1 or GBP2 reduces FRE-luciferase activity in the entire body. n = 5. One copy of UAS transgene, UAS TSC1 and UAS TSC2 (three UAS in total) were co-expressed using the C7 Gal4 driver for all experiments. For the wild-type control, we overexpressed three copies of UAS GFP using the C7 Gal4 driver. Treatments sharing the same letter indicate the groups that are statistically indistinguishable from one another (ANOVA and pairwise t tests, p < 0.05). Growth rate was analyzed by ANCOVA and post hoc comparisons of the slopes. The supplementary file in which the data used to generate each plot can be found is S1 Data.