GBP1 and GBP2 are downstream of TOR signaling.

2016-02-29T07:36:38Z (GMT) by Takashi Koyama Christen K. Mirth
<p><b>(A, B)</b> Overexpressing either GBP1 or GBP2 partially rescues body size reduction in females (A) and males (B) with reduced TOR signaling in their fat bodies. <i>n</i> = 47–64 for A and 59–67 for B. <b>(C)</b> Overexpressing either GBP1 or GBP2 partially increases growth rate in larvae with reduced TOR signaling in the fat body. <i>n</i> = 14-18/time point. <b>(D)</b> Overexpressing either GBP1 or GBP2 partially rescues the duration of the L3 in TOR signaling reduced larvae. <i>n</i> = 108–129. <b>(E, F)</b> Overexpressing either GBP1 or GBP2 reduces ILP2 (E) and ILP5 (F) accumulation in the insulin-producing cells. <b>(G, H)</b> Overexpressing GBP1 or GBP2 reduces the densities of ILP2 (G) and ILP5 (H) signals in the insulin-producing cells. We standardized the densities of ILPs by fixing the values from <i>C7</i>>GFP, TSC1, TSC2 to 1. <i>n</i> = 30–42 for both ILP2 and ILP5. <b>(I)</b> Overexpressing either GBP1 or GBP2 reduces FRE-luciferase activity in the entire body. <i>n</i> = 5. One copy of UAS transgene, UAS TSC1 and UAS TSC2 (three UAS in total) were co-expressed using the <i>C7</i> Gal4 driver for all experiments. For the wild-type control, we overexpressed three copies of UAS GFP using the <i>C7</i> Gal4 driver. Treatments sharing the same letter indicate the groups that are statistically indistinguishable from one another (ANOVA and pairwise <i>t</i> tests, <i>p</i> < 0.05). Growth rate was analyzed by ANCOVA and post hoc comparisons of the slopes. The supplementary file in which the data used to generate each plot can be found is <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002392#pbio.1002392.s001" target="_blank">S1 Data</a>.</p>