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Functionally Active Membrane Proteins Incorporated in Mesostructured Silica Films
journal contribution
posted on 2018-03-13, 13:46 authored by Justin
P. Jahnke, Matthew N. Idso, Sunyia Hussain, Matthias J. N. Junk, Julia M. Fisher, David D. Phan, Songi Han, Bradley F. ChmelkaA versatile
synthetic protocol is reported that allows high concentrations
of functionally active membrane proteins to be incorporated in mesostructured
silica materials. Judicious selections of solvent, surfactant, silica
precursor species, and synthesis conditions enable membrane proteins
to be stabilized in solution and during subsequent coassembly into
silica–surfactant composites with nano- and mesoscale order.
This was demonstrated by using a combination of nonionic (n-dodecyl-β-d-maltoside or Pluronic P123),
lipid-like (1,2-diheptanoyl-sn-glycero-3-phosphocholine),
and perfluoro-octanoate surfactants under mild acidic conditions to
coassemble the light-responsive transmembrane protein proteorhodopsin
at concentrations up to 15 wt % into the hydrophobic regions of worm-like
mesostructured silica materials in films. Small-angle X-ray scattering,
electron paramagnetic resonance spectroscopy, and transient UV–visible
spectroscopy analyses established that proteorhodopsin molecules in
mesostructured silica films exhibited native-like function, as well
as enhanced thermal stability compared to surfactant or lipid environments.
The light absorbance properties and light-activated conformational
changes of proteorhodopsin guests in mesostructured silica films are
consistent with those associated with the native H+-pumping
mechanism of these biomolecules. The synthetic protocol is expected
to be general, as demonstrated also for the incorporation of functionally
active cytochrome c, a peripheral membrane protein
enzyme involved in electron transport, into mesostructured silica–cationic
surfactant films.
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mesostructured silica filmssurfactantworm-like mesostructured silica materialsmembrane proteinsUVsilica precursor specieslight absorbance propertiesmesostructured silica materialsMesostructured Silica Filmslight-responsive transmembrane protein proteorhodopsinmembrane protein enzymeActive Membrane Proteins Incorporated
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