Functional and transcriptional differences between degranulating cells in LN and blood.

<p><b>(A)</b> Flow cytometry plots (HIV-uninfected subject) of matched LN and PB CD107a+ SEB stimulated CD4+ T cell responses. Graphs are showing the frequencies of LN (top) and PB (bottom) CD107a+ SEB stimulated CD4+ T cell responses for CXCR5-, CXCR5+ and CXCR5<sup>hi</sup> cells. <b>(B)</b> Flow plots (HIV-infected CP) illustrating the expression of CD107a+ (red) Gag-specific CD4+ T cells in relation to CXCR5 between LN (top) and PB (bottom). <b>(C)</b> Corresponding plots from the same subject showing the expression of CD107a+ (red) Gag-specific CD4+ T cells in relation to CXCR5 and perforin for LN (top) and PB (bottom). Graphs represent the frequency of CD107a+ cells within the CXCR5+, CXCR5<sup>hi</sup> and perforin+ compartment for LN (top) and PB (bottom) Gag-specific CD4+ T cells. <b>(D)</b> Biomark analysis illustrating the tSNE distribution of single SEB stimulated CD107+ CD4+ T cells from LN (black) and PB (gray). Individual graphs represent the relative Log2 expression of different markers being significantly different (P<0.05) between blood and LN CD107+ cells. Non-parametric Kruskal Wallis test with Dunn’s multiple comparison test was performed to determine significant differences between groups; *<i>P</i> < 0.05, **<i>P</i> < 0.01 and ***<i>P</i> < 0.001. All data-points are derived from the North-American and Mexico cohort.</p>