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Free Radical Scavenging Activity of 4‑(3,4-Dihydroxybenzoyloxymethyl)phenyl‑O‑β‑d‑glucopyranoside from Origanum vulgare and Its Protection against Oxidative Damage
journal contribution
posted on 2012-08-08, 00:00 authored by Chia-Hua Liang, Leong-Perng Chan, Hsiou-Yu Ding, Edmund Cheung So, Rong-Jyh Lin, Hui-Min Wang, Ying-Ging Chen, Tzung-Han Chou4-(3,4-Dihydroxybenzoyloxymethyl)phenyl-O-β-d-glucopyranoside (DBPG), a polyphenolic glycoside,
isolated
from Origanum vulgare has shown 1,1-diphenyl-2-picrylhydrazyl
(DPPH•)-scavenging capacity in previous work. This
study demonstrated that DBPG exhibits antioxidant activity by a series
of DPPH•, 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic
acid) diammonium salt (ABTS•+), and superoxide anion
radical (O2•–) radical-scavenging
assays. The inhibition of lipid peroxidation (LP) by DBPG exceeded
that by l-ascorbic acid (AA) in a liposome model system.
Adding DBPG to mouse liver and brain tissue inhibited the formation
of thiobarbituric acid reactive substances (TBARS) to a greater extent
than did trolox. In the oxygen stress test, BNLCL2 and HaCaT cells
pretreated with DBPG showed increased activities of glutathione peroxidase
(GPx), perhaps as a result of reduction of the production of reactive
oxygen species (ROS). These findings proved that DBPG had antioxidant
activities and a cytoprotective effect in hepatocytes and keratinocytes,
suggesting that DBPG may be a useful food and cosmetic additive.