Fractionation, amino acid profiles, antimicrobial and free radical scavenging activities of <i>Citrullus lanatus</i> seed protein

2017-04-10T10:24:55Z (GMT) by Priyanka Dash Goutam Ghosh
<p>In the present study, a modified Osborne fractionation method was followed to isolate albumin (<i>C</i><sub>alb</sub>), globulin (<i>C</i><sub>glo</sub>), prolamin (<i>C</i><sub>pro</sub>) and glutelin (<i>C</i><sub>glu</sub>) successively from seeds of <i>Citrullus lanatus</i> (watermelon). This research work was undertaken to investigate the antimicrobial and antioxidant activities of isolated protein fractions of <i>C. lanatus</i> seed. Amino acid composition and molecular weight distribution were determined to establish their relationship with antimicrobial and antioxidant activity. Among all the fractions, <i>C</i><sub>pro</sub> was found to be most effective against <i>A. baumannii</i> followed by <i>C</i><sub>alb</sub> and <i>C</i><sub>glo</sub>. The results showed that growth of inhibition of these protein fractions differ significantly from each other (<i>p</i> ≤ 0.05). In view of antioxidant potential, <i>C</i><sub>glo</sub> exhibited strongest antioxidant capacity while <i>C</i><sub>glu</sub> showed weakest antioxidant potential.</p>