Flow cytometric analysis of intraepithelial lymphocytes.

Proportions of TCRγδ⁺ cells among CD4^- CD8α^- (DN T cells) (A). Proportions of TCRγδ⁺ cells among all T cells (B). Gating strategy to identify TCRγδ⁺ DN T cells (C): first, all non-T cells are gated out based on lack of αβ or γδ TCR, next, CD4+ and CD8a⁺ cells are gated out, and the resulting population analyzed for expression of TCRδ and TCRαβ. Comparisons were made using a two-tailed unpaired Student’s t-test; error bars represent SEM; *: p<0.05; n = 10 (non-CD controls) and 11 (CD).