1/1
Figure S3C - ER
SIM image reconstruction and registration log files and overlay image for mSin1.2-GFP (enhanced by Atto488-conjugated anti-GFP scAb) and mCherry-tagged calnexin.
The reconstructed SIM image file was split into 2 fluorescence channels and the contrast was adjusted across the entire image using ImageJ (Fiji built). No non-linear adjustments were made. Contrast settings: 500..3000 (calnexin); 500..5000 (mSin1).
