Figure S2: No effects of doxycycline treatment on HIV-1 infection in parental U2OS cells. from Dual role of the chromatin-binding factor PHF13 in the pre- and post-integration phases of HIV-1 replication

Hofmann, Stephan; Dehn, Sandra; Businger, Ramona; Bolduan, Sebastian; Schneider, Martha; Debyser, Zeger; Brack-Werner, Ruth; Schindler, Michael

doi:10.6084/m9.figshare.5436583.v1

rsob170115_si_002.pdf (17.38 kB)

Figure S2: No effects of doxycycline treatment on HIV-1 infection in parental U2OS cells. from Dual role of the chromatin-binding factor PHF13 in the pre- and post-integration phases of HIV-1 replication

journal contribution

posted on 2017-09-25, 10:46 authored by Stephan Hofmann, Sandra Dehn, Ramona Businger, Sebastian Bolduan, Martha Schneider, Zeger Debyser, Ruth Brack-Werner, Michael Schindler

U2OS cells were treated with 1 μg/ml doxycycline or left untreated for 24 h before cells were infected with 100 ng p24 VSVG pseudotyped HIV-1 NL4-3 IRESeGFP. 24 hpi cells were analyzed by flow cytometry. The mean percentage of GFP+ cells from three independent experiments was calculated and the resulting data was normalized to untreated cells (100 %).