Figure 11. PGCLC induction is blocked by SOX17 depletion.

Figure 11. PGCLC induction is blocked by SOX17 depletion. SOX17-AID-Venus hESCs, expressing TIR1-DD either from AAVS1 or from random PiggyBac integration, were induced to form PGCLCs. Half of the cells were treated from the start of the induction with IAA and Shield1. PGCLC fraction was measured by flow cytometry after four days. Results from cells without TIR1 are plotted for comparison. Significance values are by Wilcoxon test (* p < 0.05, ** p < 0.01, ***p < 0.001).