Fig S5.tif (4.35 MB)
Fig S5. Structural modelling and active sites of the MCR complex in Ca. M. Bin16.
a, Model of the Mcrα
(red), Mcrβ (cyan) and Mcrγ (yellow) subunits of Bin16 aligned with Mcrαβγ
(violet) of Methanopyrus kandleri AV19 crystal structure (PDB owID: 1E6V). b and c, Models
of the active sites of the alpha subunit (red), beta subunit (cyan) and gamma
subunit (green) of MCR in Bin16 superimposed onto Mcrαβγ (brassiness) of Methanopyrus kandleri AV19. Backbones of
the complex in close proximity to ligand binding sites are shown. Cofactor F430
(gray), coenzyme M (yellow) and coenzyme B (steel blue) are shown as balls and sticks. The side chains
of residues interacting with the cofactors are shown with hydrogen atoms
masked.
