Fig S3 from Role of long-chain acyl-CoAs in the regulation of mycolic acid biosynthesis in mycobacteria

<b>Effect of C<sub>26</sub>-CoA on the formation of C<sub>20</sub>-CoA/ His<sub>6</sub>-MabR<sub>MT</sub> /P<i>fasII</i> complex.</b> His<sub>6</sub>-MabR<sub>MT</sub> was preincubated during 5 min with C<sub>26</sub>-CoA in the presence of poly-dIdC, prior to the addition of C<sub>20</sub>-CoA and 5 min later, P<i>fasII</i> probe was added (last 2 lanes). The His<sub>6</sub>-MabR<sub>MT</sub>-DNA complex formation was also assayed with the individual addition of different concentrations of C<sub>20</sub>-CoA (lanes 3-6) or C<sub>26</sub>-CoA (lanes 7-10), as controls of the experiment.