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Ferromagnetic Resonance Biosensor for Homogeneous and Volumetric Detection of DNA
journal contribution
posted on 2018-05-31, 00:00 authored by Bo Tian, Xiaoqi Liao, Peter Svedlindh, Mattias Strömberg, Erik WetterskogThe
ability to detect and analyze the state of magnetic labels
with high sensitivity is of crucial importance for developing magnetic
biosensors. In this work, we demonstrate, for the first time, a ferromagnetic
resonance (FMR) based homogeneous and volumetric biosensor for magnetic
label detection. Two different isothermal amplification methods, i.e.,
rolling circle amplification (RCA) and loop-mediated isothermal amplification
(LAMP), are adopted and combined with a standard electron paramagnetic
resonance (EPR) spectrometer for FMR biosensing. For the RCA-based
FMR biosensor, binding of RCA products of a synthetic Vibrio cholerae target DNA sequence gives rise to
the formation of aggregates of magnetic nanoparticles. Immobilization
of nanoparticles within the aggregates leads to a decrease of the
net anisotropy of the system and a concomitant increase of the resonance
field. A limit of detection of 1 pM is obtained with a linear detection
range between 7.8 and 250 pM. For the LAMP-based sensing, a synthetic
Zika virus target oligonucleotide is amplified and detected in 20%
serum samples. Immobilization of magnetic nanoparticles is induced
by their coprecipitation with Mg2P2O7 (a byproduct of LAMP) and provides a detection sensitivity of 100
aM. The fast measurement, high sensitivity, and miniaturization potential
of the proposed FMR biosensing technology makes it a promising candidate
for designing future point-of-care devices.
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Keywords
RCA-based FMR biosensorEPRnanoparticle1 pMdetection rangedetection sensitivityFerromagnetic Resonance Biosensoramplification methodsZika virus target oligonucleotideresonance fieldcircle amplificationRCA productsFMR biosensing technologyMg 2 P 2 O 7future point-of-care devices250 pMVolumetric Detectionlabel detectionFMR biosensingaggregateVibrio cholerae target DNA sequence100 aMImmobilization
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