FASN induces H3K9me3 to repress CRISP1 expression.

(A) ChIP-seq was performed in SK-UT-1-EV and–FASN cells with anti-H3K9me3 antibody. Enriched genomic regions were compared between SK-UT-1-FASN and SK-UT-1-EV. Screenshot shows CRISP1 is enriched for H3K9me3 in SK-UT-1-FASN cells (solid line) vs. SK-UT-1-EV (dot line); (B) Enrichment of CRISP1 binding to SK-UT-1-FASN genomic DNA by ChIP-PCR. Immunoprecipited chromatin DNA by H3K9me3 antibody was reversed crosslinking, purified and subject to q-PCR for CRISP1 gene. (C) qRT-PCR for CRISP1 expression. SK-UT-1-EV or–FASN cells were lysed for total RNA extraction, cDNA synthesis and q-PCR of CRISP1. *, p<0.05 FASN-transduced SK-UT-1 vs. EV-transduced SK-UT-1 cells. Data was normalized to GAPDH or β-actin. Results are expressed as the percentage of input DNA.