(A) Expression of Derlin- protein using western blotting in SV-HUC-1 cells, um-uc-3 cells and T24 cells. (B) Interfere of Derlin-1 in T24 cells. Cells were transfected with nontargeting siRNA or Derlin-1 siRNA for 48h, and the protein expression was detected by western blotting. NC, siRNA negative control; siRNA, Derlin-1 siRNA. Data are expressed as the mean ± S.D., **P < 0.01, ***P < 0.001.