Examples of candidate biomarkers.

(A) Scatterplot showing the discrimination of the early infection and control groups using m/z 3720 at F6ISL (Fraction 6, IMAC chip, Low laser intensity). Blue dots: control. Red dotes: early stage of infection. (B) Scatterplot showing the discrimination of the acute and chronic groups using m/z 13407.2 at F6CSH (Fraction 6, CM10 chip, High laser intensity). Blue dots: acute. Red dotes: chronic. (C) Example of a candidate biomarker increased in infected mice over time. Serum SELDI-TOF MS mass spectra obtained for F6ISL (Fraction 6, IMAC chip, Low laser intensity) from infected mice (top 3 spectra) versus non-infected mice (bottom spectrum). A candidate biomarker as 7081 Da is gradually up-regulated from 3, to 6, to 12 weeks after infection (P value 0.006). (D) Example of a candidate biomarker increased in infected mice regardless the parasite burden. Serum SELDI-TOF MS mass spectra obtained for F6CSL (Fraction 6, CM10 chip, Low laser intensity) from infected mice (bottom 4 spectra) versus non-infected mice (top spectrum). A candidate biomarker as 5566.31 Da is up regulated in infected mice dependent on the infection rather than the dose of infected agents (P value 0.006). G1 infected with 200 cercariae, G2 infected with 150 cercariae, G3 infected with 100 cercariae, G5 infected with 50 cercariae.