Evaluation of signaling pathways activated by R-568 in MVB from WKY and SHR.

<p>MVB homogenates from experiments described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0202354#pone.0202354.g001" target="_blank">Fig 1</a> were subjected to immunoblotting for total and phosphorylated forms of Akt (phospho-Akt<sup>Ser473</sup>), and eNOS (phospho-eNOS<sup>Ser1177</sup>). Total β-actin levels were used as loading control. A representative set of immunoblots is shown for experiments that were repeated independently 3 times. Each bar represents the mean ± SEM of densitometric analysis for phosphorylated proteins normalized to their respective total forms. *p < 0.05 <i>vs</i> respective basal.</p>