Evaluation of endothelial mediators involved in vascular effects of R-568 in MVB from WKY and SHR rats.

<p>(A) and (B) Representative tracings of vascular response to R-568 (10 nM—30 μM) in MVB from WKY (A) or SHR (B) pretreated with L-NAME, CTX and INDO are shown. Each symbol represents the start of a 4-min perfusion. (C) MVB isolated from 8-week old WKY (closed symbols) were stimulated with increasing concentrations of R-568 in the absence (circles) or in the presence of L-NAME (100 μM/20 min; squares), L-NAME+CTX (300 nM/20 min; triangles), L-NAME+CTX+INDO (10 μM/20 min; diamonds). Results are the mean ± SEM of 4 WKY independent experiments. (D) MVB isolated from 8-week old SHR (open symbols) were stimulated with increasing concentrations of R-568 in the absence (circles) or in the presence of L-NAME (100 μM/20 min; squares), L-NAME+CTX (300 nM/20 min; triangles), L-NAME+CTX+INDO (10 μ M/20 min; diamonds). Results are the mean ± SEM of 4 WKY and 4 SHR independent experiments. *p < 0.04 and <sup>#</sup>p < 0.05 <i>vs</i> respective CTRL curve.</p>