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Evaluation of Drug Exposure and Metabolism in Locust and Zebrafish Brains Using Mass Spectrometry Imaging
journal contribution
posted on 2018-01-19, 12:50 authored by Marvin Villacrez, Karin Hellman, Tatsuya Ono, Yutaka Sugihara, Melinda Rezeli, Fredrik Ek, Gyorgy Marko-Varga, Roger OlssonStudying how and
where drugs are metabolized in the brain is challenging. In an entire
organism, peripheral metabolism produces many of the same metabolites
as those in the brain, and many of these metabolites can cross the
blood-brain barrier from the periphery, thus making the relative contributions
of hepatic and brain metabolism difficult to study in vivo. In addition,
drugs and metabolites contained in ventricles and in the residual
blood of capillaries in the brain may overestimate drugs’ and
metabolites’ concentrations in the brain. In this study, we
examine locusts and zebrafish using matrix assisted laser desorption
ionization mass spectrometry imaging to study brain metabolism and
distribution. These animal models are cost-effective and ethically
sound for initial drug development studies.